High specificity to a single epitope

         

Low likelihood of cross-reactions and background noise

         

Batch to batch homogeneity

         

Easy to produce large quantities of identical antibody

         

Higher affinity against the antigen due to recognition of multiple epitopes

         

Tolerance to antigen changes

         

Easy to produce with an inexpensive price and a short period of time

         

Highly stable and tolerant of pH or buffer changes

         

Detection for a specific antigen

         

Staining cells with less background

         

Detection of the changes in molecular conformation or phosphorylation states

         

Immunotherapy

         

Detection for a known or unknown isoforms of antigens with high antigen homolog

         

Detection for targets with possible genetic glycosylation or conformational changes

         

Detection a target in solutions with varying pH and salt concentrations

         

Can be used to produce Monoclonal Antibodies

         

Short immunization cycle

         

Low cost

         

Little antigen required

         

Good for small amount of polyclonal antibody production

         

Low cost

         

Small amount of serum

         

High specificity

         

Moderate cost

         

Good alternative for highly conserved mammalian proteins

         

Difficult to isolate antibodies