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Phospho-Specific Antibody Services
Phosphorylation of proteins plays an important role in many key regulatory mechanisms of life phenomena such as signaling, cell cycle, proliferation, metabolism, growth & development and cancer mechanisms.
Phosphorylation process is usually the addition of a phosphate (PO4) group to a protein, commonly at a serine (S), threonine (T) or tyrosine (Y) residue via the action of a kinase. Phosphorylation-Specific Antibody can discriminate between the phosphorylated and the non-phosphorylated state of a protein. It is one of the most frequently asked for types of PTM specific antibodies.
Design and synthesis of phosphopeptide
10mg phosphopeptide
5mg non-phosphopeptide
(synthesized by TGpeptide)
Peptide conjunction
Couple peptides to carrier(KLH, ABS etc.)
Production of anti-serum
Pre-immune bleed, Bleed after four times of Immunization
Miniature purification
Collecting and test of serum titer
Antibody affinity purification
Antibody affinity purification
QC analysis
Analysis by SDS-PAGE and UV ELISA validation
Final Deliverables:
Antiserum or 1~3 mg phospho-specific antibody
1~2 ml of pre-immune serum
2~4 mg phosphopeptide（>90% purity)
2~4 mg non-phosphopeptide（>90% purity)
Experimental report
Design and synthesis of phosphopeptide
10mg phosphopeptide
5mg non-phosphopeptide
(synthesized by TGpeptide)
Peptide conjunction
Couple peptides to carrier(KLH, ABS etc.)
Immunization and test
Immunization and make ELISA test on phosphopeptide and non-phosphopeptide
Fusion and screening
• Fusion and screening
• Subcloning and cell expansion
• Cell Banking
Antibody production and analysis
• Hybridoma cell culture at a proper scale
• Purified by protein A affinity chromatography
QC analysis
Analysis by SDS-PAGE and UVELISA validation
Final Deliverables：
50ul of pre-immune serum
2~4 mg phosphopeptide（>90% purity)
2~4 mg non-phosphopeptide（>90% purity)
Experimental report
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