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Bacterial expression
E.coli is the preferential one of bacterial systems for recombinant protein production and large scale fermentation as its advantages including fast rate of reproduction, ease of culture, and rich knowledge about its genetics. With abundant experience of soluble protein expression and production and protein refolding, TGpeptide can provide you high-quality expression service.
1.Gene synthesis and codon optimization (optional)
1-2 weeks
2.Vector construction
1-2 weeks
3.Expression evaluation
1-2 weeks
4.Protein expression and purification
2-3 weeks
Deliverables:
TGpeptide provide endotoxin content testing service. Endotoxins or LPS are cell membrane components of Gram-negative bacteria (such as E. coli). A single E. coli cell contains about 2 million LPS molecules. At the stage of actively growing, E.coli cell sheds small amounts of endotoxins into its surroundings, however, large amounts can be released while it dies.
Vitro enzyme activity experiment
No
Production for antigen
No
Vitro cell culture
<1EU/ug
Other animal vivo experiment
<1EU/ug
The detection of endotoxin performs before and after endotoxin removal. The primary methods can be divided into qualitative (LAL gel-clot assay) or quantitative (LAL turbidimetric assay and LAL chromogenic assay).
E.coli
1 million EU/ml
Running water
10-100 EU/ml
Biologic pharmacy
<0.01 EU/ml
Intrathecal injection drugs
0.2 EU/kg
Non-intrathecal injection drugs
<5 EU/kg
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