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Insect Expression

     The baculovirus expression vector system (BEVS) is one of the four major expression systems of genetic engineering. BEVS has several advantages of using the baculovirus-insect cell expression system, such as improved solubility and comprehensive post-translational modifications.

Service procedures

Specification

Timeline

1.Analysis of gene and target protein

Gene analysis

Codon optimization

         

Gene synthesis

1-2weeks

2.Construction of expression vector

Subcloning to expression vector

Plasmids Prep and analysis

         

Amplification of recombinant plasmids

1-2weeks

3.Screening of expression strains

Subcloning to baculovirus

Amplification of recombinant baculovirus

2-3weeks

4.Expression optimization and amplification of baculovirus-insect cells

Baculovirus infecting cells

Expression optimization

Amplification

2-3weeks

5.Purification and detection

Purification and condition optimization

SDS-PAGE and UV analysis

3-4weeks

  • Deliverables

    Sequencing report

    Feasibility report

    0.1-0.5 mg sample (If expression is feasible and sample remains from QC testing)

    Purified protein

    SDS-PAGE report

    CoA

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