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Mammalian expression
Mammalian cells can correctly and effectively express eukaryotic proteins, including the synthesis, processing, and secretion of proteins. The mammalian system has the excellent function of post-translational modification of proteins and can assemble proteins correctly. Our Mammalian expression can provide flexible services which could meet different custom needs, including tag removal, endotoxin control, SEC-HPLC assay, glycosylation analysis and so on.
Mammalian cells are capable of protein folding and post-translational modification, which can express proteins with molecular structures, physical and chemical structures, and biological functions mostly approaching natural higher-orgainism proteins. It brings more possibility to obtain the same bioactivity as natural protein. Cells that are frequently used for transient transfection are HEK293 or CHO cells. Large volume transient transfection of HEK293 and CHO cells adapted to suspension culture has addressed the need to obtain amounts of recombinant protein without the time-consuming and labor-intensive creation of stable cell lines.
Gene synthesis and codon optimization (optional)
1-2 weeks
Vector construction
1-2 weeks
Expression and purification
2-3 weeks
Large-scale expression and purification (optional)
2-3 weeks
Deliverables:
Stable cell line is widely used in various areas, such as recombinant protein and antibody production, testing and analysis, gene editing, functional study and so on. Engaged in protein expression study for years and possessing abundant stable cell line development experience, TGpeptide can increase stable cell line productivity and improve experimental results.
Testing
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Plasmid construct
3 weeks
Transfection
1 week
Screening
cell pools: 4weeks
single clones: 8weeks
Amplification(optional)
2-3 months
QC
2-10 weeks (According to your requirement)
Notes:
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