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Yeast Expression
Yeast protein expression system is a ideally suitable expression system for both secretion and intracellular expression as the yeast offer relatively easy genetic manipulation and rapid growth to high cell densities on inexpensive media. Common yeast protein expression systems are Pichia pastoris, Saccharomyces cerevisiae, Kluyveromyces lactis, Hansenula polymorpha, and Yarrowia lipolytica. TGpeptide use Pichia pastoris as our strain.
1. Analysis of gene and target protein
1-2 days
2.Subcloning
3.Screening of expression strains
3-4 weeks
4.Protein expression and purification in large scale
2-3 weeks
5.Screening of expression strains
3-4 weeks
Deliverables:
Expression vector containing the target gene
Target sequencing report.
E.coli containing plasmids of the expression vector
Plasmid instructions
SDS-PAGE analysis results
Expression strain (extra charge, $1500 / strain)
Purified recombinant protein (purity >90%)
Related analysis reports
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